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Magnetofection combines chemical and physical approaches. This involves combining nucleic acids, transfection reagents (polymers, lipids) or viruses with magnetic nanoparticles to improve transport of the material to the cell, directed by a specific magnetic field.
Specificities
:
• Increased transfection efficiency
• Low toxicity because it is better absorbed by the cells, so less material is needed
• A simple, effective protocol
• High transgene expression
Applications :
• Suitable for primary cells and cells that are difficult to transfect
• Suitable for all nucleic acids:
• DNA, oligonucleotides, mRNA, siRNA, shRNA
• Not suitable for cells in suspension
• Compatible with the use of serum
