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• Kit for the preparation of NGS libraries for Illumina® sequencer
• Patented RC-PCR ("reverse complement PCR") technology:
- Ligation of adapters and enrichment by multiplex PCR in a single tube
- Simple, rapid and economical protocol applied directly from purified nucleic acids (no fragmentation or end repair required)
- Reduced risk of off-target binding and cross-contamination
- Highly sensitive : low amount of starting DNA/cDNA required
• Kit supplied with RC-PCR probe mix, RC-PCR HiFi master mix and RC-PCR probe dilution buffer
• To be completed with Primer Index plates (containing pre-distributed primers)
Specificities
:
• For forensic medicine applications: identification of persons, determination of parentage, linking persons of interest to crime scenes, etc.
• 96 reactions formats
• Kits 316247, 316250 and 316251 are to be completed with one Index Primer plate, kits 316248 and 316249 are to be completed with two Index Primer plates.