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• Reaction mixtures for RT-qPCR in one step
• Contains all components necessary for cDNA synthesis and probed qPCR (besides matrix, primers and probes) in a single tube
• Probe Kit optimized for singleplex and duplex (1 to 2 targets)
• Multiplex Probe Kit Optimized for Multiplex (up to 4 targets)
• Optimized for DNA and LNA probes
• Probe Kit contains ROX dye for standardization
Specificities
:
Probe Kit consisting of three reagents• Mix qPCR 5x with DNA polymerase HOT FIREPol, reaction buffer, dNTPs (without dUTP), 15 mm MgCl2 (final concentration 3 mm), ROX dye
• Mix SOLIScript 40x with SOLIScript reverse transcriptase and RIBOGRIP ribonuclease inhibitor
• Molecular biology quality Water