The BIORCELL3D® consortium brings together researchers in Biology and Pharmaco-chemistry from the IMoST research team (UMR INSERM-UCA 1240) and specialists from the Biotechnology industry from BIOPASS and BIOMARQUEURS (Clermont-Ferrand - 63).
Together, they develop and sell innovative preclinical biotechnology tools applied to Health and the Environment, such as :
Artificial Tumours® (3D cultures) for new cancer drug screening. These preclinical models are derived from well-known tumor cell lines widely used by researchers and faithfully reproduce avascular micro-tumors. They are available as a service or as ready-to-use kits (Opti3D® kits).
Serum-free and chemically defined culture media as alternatives to calf serum, allowing 2D and 3D cell culture of a wide range of cell types (healthy and tumorous), as well as short-term preservation of cell samples without freezing (OptiPASS® media range).
Fluorescent reagents capable of measuring the level of resistance of tumor cells following anticancer treatments in a rapid and dynamic manner (Patent registered LightSpot® by UCA-BIOMARQUEURS).
Services: custom development of new 3D models, screening of molecules (drug screening), or efficacy tests using artificial tumors.
Artificial Tumor Kits® in ready-to-use microplates for in vitro testing
Artificial Tumors® (3D cultures) from tumor cell lines widely used in oncology, and in particular derived from aggressive triple-negative breast cancers.
Complete, ready-to-use serum-free cell culture media
OptiPASS® serum-free and chemically defined media can be used for the culture of tumorous or healthy cells, in monolayer (2D culture) and 3D culture (spheroids, organoids) of a wide range of tumour types of interest in preclinical research.
2D Culture (tumorous and healthy cells) and Bioproduction
For 2D culture, the OptiPASS® medium allows :
Non-exhaustive list of tumour and healthy lines validated in 2D culture (OPTI03):
(Requires prior coating of culture plates, please refer to the technical department for further information and support)7
3D culture ( tumorous and healthy cells)
Benefits :
List of tumour lines validated in 3D culture:
Oncology-preservation
Once produced, the 3D cultures can be stored for several days in OptiPASS® (OPTI05), under anoxic conditions and at 4°C before being analyzed.
Examples of tumor lines validated with this method: breast: MDA-MB-231, SUM1315.
Innovation improving the detection, localization, and quantification of Multidrug Resistance proteins in all cellular samples.
Applications:
Aims:
Available for different excitation/emission wavelengths.
Development of new 3D tumor cell models on demand.
Development of new 3D tumour cell models on demand.
Presentation of the screening service
For more details on Biocell3D® products and services, brochures are available for download.
LightSpot®Artificial Tumour Kits® and serum-free culture media
