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Molecular biology Electrophoresis Electrophoresis products and reagents

Electrophoresis products and reagents

Agarose Low EEO Ozyme ClearLine®

• Agarose à faible électroendosmose (Low EEO)
• Idéal pour toutes les applications courantes d'électrophorèse (analytique et préparative)
• Résolution fine de l'ADN
• Exempt de DNase/Rnase

Electrophoresis / DNA ladders

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EMBITEC landscape gel

• 1% Agarose TAE with GelGreen (alternative solution to BET) landscape gel
• Fragments optimal separation: 600 to 10000 bp
• 6 mm gel thickness
• Utilisable avec la cuve RunOne
• Convenient: place gel with tray directly onto electorforesis platform
• Safe: no UV light necessary for viewing gels with GelGreen. Simple waste treatment procedure (elimination with organic waste)

Agarose tablets

• Agarose with low EEO for a rapid separation of DNA and RNA fragments
• Allows to use gel concentrations from 0.75 % - 2 %, guarantees separation efficiency from 250 bp to 23 kb
• Soak in buffer for 2 minutes before heating
• Provides time savings and reproducibility for gelling

Molecular biology grade powder agarose

• Ultra-pure agarose, low background noise
• Easy to dissolve
• Utilisable to faible concentration (0.75 - 2 %)
• Separation efficiency from 250 pb to 25 kb
• Gel strength: >1000g/ cm² (1%), >2000g/ cm² (1.5%)
• Clarity:< 4 NTU
• DNase/RNase free

Agarose Basic

• For the separation of DNA and RNA fragments and may be used at concentrations between 0.8 and 2 %

Agarose Condalab for Molecular Biology

Specific products for molecular biology:
• Standard agarose used in electrophoresis techniques;
• High Resolution Agarose;
• Agarose with a low melting point for DNA recovery;
• Dehydrated media for molecular biology;
• Acrylamides in solution and buffers.
• DNAs and RNAs free

Agarose

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Agarose Tablets, TBE and Midori Green Advance

Midori Green Advance is a non-carcinogenic alternative to ethidium bromide for the labeling of nucleic acids.

Agarose

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Agarose Tablets

• Agarose with low electroendosmosis for DNA or RNA separation
• Allows use of concentrations from 0.75 - 2%. Separation efficiency from 250 bp to 23 kb guaranteed
• Initial tablet dissolution: 2 min at room temperature
• Take one tablet (0.5 g) and add 50 ml gel running buffer, the result is a 1% gel

Ethidium bromide dropper bottle

For easy and safe addition of ethidium bromide in solutions. Add one drop per 50 ml of solution to obtain a concentration of 0.5 µg / ml. Bottle is waterproof and provides protection against UV.

Ethidium bromide

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Instastain® ethidium bromide staining card

• Quick and easy
• No pipetting required, delivers a small amount of stain to the agarose
gel via a special paper backing
• Place the card on the gel and wait: 8-10 min. for 1% agarose gels and 3-5 min. for 0.8% agarose gels
• Each card will stain a gel up to 7 x 7 cm in size

ClearLine® EmeralDye Stain

High sensitivity even for small fragments, superior signal to noise ratio, can be used in a classical gel staining procedure as well as in post staining procedures, 4 - 6 µl in 100 ml of agarose.

Midori Green stains

• For the labeling of nucleic acids
• Non-carcinogenic alternative to ethidium bromide
• Storage solution without DMSO

3 versions:
• Midori Green Advance: high sensitivity for small fragments, background noise reduction, dye to be added during the preparation of the gel or following the migration, 4 - 6 µl for 100 ml of agarose; main excitation wavelength 490 nm / secondary 290 nm; emission wavelength 530 nm
• Midori Green Direct: mix directly with the samples ratio 10:1 , background noise reduction, included a dye load; main excitation wavelength 490 nm / secondary 270 nm; emission wavelength 530 nm
• Midori Green Xtra: ultra sensitive dye when excited in blue or blue / green light (detecting nucleic acids aquantity detection lower than 4 ng), for excitation in the UV rather choose the Midori Green Advance, very low background noise, 4 - 8 µl for 100 ml of agarose; main excitation wavelength 482 nm / secondary 250 nm; emission wavelength 509 nm

DNA-dye NonTox

• Non-hazardous, non-mutagenic and non-toxic alternative to ethidium bromide
• Ready-to-use 6X charge buffer for direct mixing with samples
• Contains 3 migration dyes: bromophenol blue, xylene cyanol and orange G
• Excitable by UV and blue light
• Excitation/emission spectrum: 495 (pic 1), 260 (pic 2) nm / 540 nm

GelRed Nucleic Acids Dye

• Stable and sensitive dye for nucleic acids visualization (RNA and single and double stranded DNA)
• Alternative to the use of BET, safe for the environment
• Presents the same excitation and emission spectra as BET
• GelRed stained nucleic acids can be used in cloning and sequencing
• 10000 X in water concentration dye
• Available in three packs: 0.1; 0.5 and 10 ml
• Main excitation wavelength 290 nm / secondary 520 nm
• Emission wavelength 595 nm

Gel Green nucleic acid gel stain

• For staining nucleic acids
• Alternative non mutagenic non cytotoxic and no ethidium bromide
• More sensitive than ethidium bromide and SYBR Green
• Extremely stable: long-term storage at room temperature and can be microwaved
• Dye to be added during the preparation of the gel or staining afterwards
• Easy to use
• Main excitation wavelength 505 nm / secondary 270 nm
• Emission wavelength 530 nm

DNAzure blue Nucleic Acid Gel Stain

• Deep blue bands visible by the naked eye following 5-30 min light exposure
• Ultrasensitive detection, as little as ~1 ng DNA
• Simplified DNA band excision, without the need for DNA damaging UV light
• Compatible with downstream applications such as sequencing and cloning
• We advise the use of orange G rather than the use of blue bromide
• Photo capture possible without imager

SmartGlow stain

• Direct replacement for Ethidium Bromide
• Excitation with both UV (290 nm) and Blue Light (490 nm)
• Store at 4 °C
• Better sensitivity than EtBr

Agarose Tablets, TBE and Midori Green Advance

Midori Green Advance is a non-carcinogenic alternative to ethidium bromide for the labeling of nucleic acids.

FastGene Precast Polyacrylamide Gel for vertical electrophoresis

• High Resolution and reproducible
• Polyacrylamide fixed or gradient concentration
• 12-well format, 45 µl per well
• 10 x 10 cm Gels compatible with Hoefer SE260, Lonza PAGEr Minigel and Life Technologies XCell Surelock
• 10 x 8 cm Gels compatible with Hoefer SE250 and SE260, Nippon Genetics FastGene Protein Chamber, Biorad Mini Protean 2, 3 and Tetra System
• Use with MOPS buffer
• Stockage: 2 to 8 °C

Acrylamide / bisacrylamide 19: 1

Ready to use solution 40%. Acrylamide / bis - acrylamide consists of 38% acrylamide and bis - acrylamide 2%. The solution is optimised for electrophoresis of nucleic acids.

Acrylamide / Bisacrylamide 37.5:1

Ready to use solution 40% (38.96% acrylamide, 1.04% bisacrylamide). This concentration makes the solution an ideal choice for the resolution of high molecular weight protein separation.

Acrylamide / Bis - Acrylamide 19:1

Ready-to-use solution 40%. Acrylamide / bisacrylamide consists of 38% acrylamide (v) and 2% bis (acrylic acid) (v).

Acrylamide / Bis - Acrylamide 37.5:1

Ready-to-use solution 40% (acrylamide 38.96%, bis-acrylamide 1.04%). This concentration makes the solution an ideal choice for solving small macromolecules.

Agarose for protein electrophoresis

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Ammonium persulphate

Initiates the polymerization of acrylamide gels. Used with TEMED.

Tween® 20

Blocking agent. Nonionic detergent used in solutions.

Ammonium persulphate

Ammonium persulphate initiates the polymerization of the acrylamide gels. Used with TEMED.

Ready-to-use buffer solutions

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Biosolve TAE buffer concentrate

The buffer TAE (Tris-Acetate-EDTA) is used in agarose gel electrophoresis for applications requiring high resolution and separation under a low voltage for high molecular weight double stranded DNA. The solutions are aseptically microfiltered.

Concentrated TAE buffer for electrophoresis gel

Ready to use. Calibrated to provide clear bands.

Buffer solutions for molecular biology

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TBE buffers

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Biosolve TBE concentrated buffer

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Buffer sachet

• Dissolve in pure water to the indicated final concentration
• Makes 10L of 1 X solution: 1 x contains: 89 mm Tris, 89 mm borate, 2 mm EDTA, pH 8.2 -8.4

TRIS

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EDTA

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Corning® Media buffered saline solutions

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Nucleic acid loading buffer

• Calibrated to provide clear bands
• Concentrated for reducing the load volume
• For DNA and RNA run on polyacrylamide and agarose gels

PCR grade prealiquoted water

• Ultrapure, sterile, deionized, double destiled and prealiquoted water
• Assayed for absence of contaminating DNA from bacterial, fungi and human sources
• DNase free

PCR tested water, RNase- and DNase-free

• Bacterial DNA: not detectable (min. 40 PCR cycles)
• DNases/RNases: not detectable
• In 1.7 ml tubes

Hyclone water for molecular biology

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Sterile, bi-distilled, non-pyrogenic water

• OTEC range
- Directives and standards
Aguettant OTEC water is an Active Pharmaceutical Ingredient (API) (Used as a pharmaceutical primary material excluding injectable, ophtalmic or inhaled products). Complies with directives NF EN ISO 3696 type 3 and European pharmacopeia's monograph «purified water in container ».
Monograph « sterile purified water » American pharmacopeia (excluding non-sterile 10 litres container)
- Uses
For rinsing and irrigation of materials and tools, white rooms cleaning, machine washing , and acting as an active substance for the manufacture of medicaments.
OTEC range cannot cannot be used neither in the hospital sector, nor for rinsing and irrigation of wounds.

• VERSOL range
- Directives and standards
VERSOL sterile non pyrogenic water complies with P.P.I and the current European pharmacopeia's monographs. In compliance with DM IIa (sterile), ISO 9001 and NF EN ISO 13485 certifications.
- Uses
These flasks are used for rising and irrigation of wounds; they are sterile and of single use. Must not be used for injections.
- Documents provided upon request (analysis bulletin)

Pure water

• Pyrogenic
• Purified water, reverse osmosis, demineralised
• Resistivity > 0.5 MΩ. cm
• Compliant with current European Pharmacopoeia - Monograph Purified water packaged in container
• Compliant with current US Pharmacopeia - Purified water monograph
• Complies with NF ISO3696 standard type 3
• Bacteriological quality < 10UFC/100 ml
• Analysis Bulletin on request
• Applications:rinsing the medico-surgical during disinfection protocols that do not require sterile water, final rinsing of laboratory instruments, cleaning in clean room, solutions preparation for PLC, buffer solutions realisation

Sterile sodium chloride Miniversol

Composition: NACl 0.9 % non-apyrogenic.

Packaging: the Miniversol 0.9 % sterile sodium chloride for rinsing and irrigation is sold in volumes from 45 ml to 1 L. The batch number and expiry date are indicated on the flask labels. Guaranteed for more than one year.

Directions and recommended uses: physiological solution for rinsing, irrigation of the skin, wounds and surgical cavities and general laboratory use. Use as primary material for the manufacture of non-injectable medicines, culture media etc.

Documents provided on request: conformity assessment to the applied European Pharmacopeia's monograph ''purified water packaged in can'' and to the ISO 3696 type 3 norm.

DNA dye for DNAzure blue gel

• Dye visible to the naked eye after 5 to 30 minutes of exposure to light
• Ultrasensitive, band visualization up to 1 ng of DNA
• Excision of the bands of interest without the use of a UV lamp
• Dye compatible with subsequent use of DNA for sequencing and cloning
• The use of bromophenol blue (found in some loading buffers) is not recommended, it is preferable to use G
• Picture taking possible without imager

Green Gel Dye

• For nucleic acid labelling
• Non-mutagenic and non-cytotoxic alternative to ethidium bromide
• More sensitive than ethidium bromide or SYBR GREEN
• Dye to be added after migration
• Main excitation wavelength 500 nm/secondary between 250 and 300 nm
• Emission wavelength 530 nm