Blotting -

Whatman blotting paper

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Nytran N nylon blotting membrane

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• Suitable for applications that require a lower charge
• Designed for Southern and Northern blotting, colony and plaque lifts and Dot-/Slot-Blots
• Compatible with isotopic and nonisotopic detection methods
• Excellent signal-to-noise ratios
• Moderate positive charge
• Consistent membrane morphology
• High sensitivity
• Nytran binding capacity: >400 µg/ cm²
• It is available in 0.2 µm and 0.45 µm pore sizes for optimal retention of oligos and larger DNA fragments

Nytran SuPerCharge (SPC) nylon blotting membrane

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• Very high positive charge, low background
• Nytran SuperCharge membrane binds nine times more molecules than a conventional nylon membrane
• The great nylon density (compared to typical nylon membranes) provides increased binding sites for your samples
• Excellent sy mmetry: gives the membrane the ability to lie flat
• Very uniform pore size and pore distribution compared to typical nylon membranes: lead to greater reproducibility of results across a membrane and from blot to blot

Amersham™ HYBOND blotting membrane

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Hybond-N
• Strong, supported membrane
• For nucleic acids blotting
• Binding capacity up to 600 µg/ cm²
• Recommended for radioactive blotting
• Nucleic acids can be crosslinked to nylon using UV ligh

Hybond-N+
• For nucleic acids blotting
• Binding capacity up to 600 µg/ cm²
• For use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems

Hybond-NX
• For nucleic acids blotting
• Binding capacity up to 600 µg/ cm²
• Developed specifically for use with low hybridization buffer volumes and high-throughput applications
• Gives clean backgrounds

Hybond-XL
• For nucleic acids blotting
• Binding capacity up to 600 µg/ cm²
• Efficient binding of small fragments
• Positively charged nylon membrane which is designed to give optimum signal-to-noise ratios when used with radioactively labeled probes

More dimensions available: please contact us.

Amersham ECL and MP Hyperfilm™

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For detection of the chemiluminescence signal of the nucleic acid and protein blots.

TurboBlotter: rapid blotting device for transfer of DNA and RNA.

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• Uses capilarity combined with gravity
• Compact: small footprint, stackable
• Blotting device for high-resolution transfer of DNA and RNA
• Rapid: downward capillary transfer allows for alkaline buffer transfers in 1 h and neutral (SSC) transfers in 3 h
• Economical: Reusable blotting device requires less buffer and blotting paper
• Easy to use: Very easy to set up and works without power or vacuum source

Each TurboBlotter Kit contains:
• 1 transfer device;
• membranes and papers, enough for five transfers

Each TurboBlotter refill contains five sets of membranes and papers, enough for five transfers.

Biosolve SSC buffer 20X concentrate

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SSC (Sodium citrate-Sodium chloride buffer) buffer is commonly used in the transfer, blocking and hybridization as well as the post-hybridization washing steps in both Northern and Southern blotting.