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Molecular Biology - Nucleic Acids PCR in end point Standard Routine DNA polymerases

Standard Routine DNA polymerases

Biodyne enzymes

• All enzymes produced are stable at ambient temperature
• A specific genetic modification: "Stability TAG" increases polypeptide shelf-life and its tolerance for temperature at a wider scale without compromising the properties of the polypeptide itself
• After being stored at ambient temperature (tested up to +35 °C), the enzyme remains fully active

Advantages
• Easy Pre-PCR preparation, fast and without any particular precautionary measures (no need for ice bath)
• The best quality/price ratio on the market
• Reduced waste and environmental impact (no need for ice shipping, smaller packaging)

Cytiva PCR components

Taq DNA Polymerase (cloned): 1 unit incorporates 10 nmol of total nucleotides into acid-insoluble material in 30 min at 70ºC, in a total volume of 50 µl.

Cytiva illustra™ PCR kits: Ready-To-Go™ technique

PuReTaq Ready-To-Go PCR Beads are pre-mixed, predispensed, single-dose reactions optimised for performing standard PCR amplifications.

Routine enzyme

• Fragment amplification up to 4 kb fidelity comparable to Taq, suitable for TA-cloning

Routine enzyme

• Fragment amplification up to 4 kb fidelity comparable to Taq, suitable for TA-cloning

Thermoprime

• Amplification of fragments from 12 kb
• Allows to make 4000 reactions of 25 µl each

Thermo-Start DNA Taq Polymerase

• Requires an activation step at 95 °C for 15 minutes
• Inactive at room temperature
• Amplifying fragments up to 5 kb
• Contains
• Thermo start (5U/µl) 10 x 50 µl
• Buffer (10X) 10 x x1.5 ml
• MgCl₂ (25 mm) 10 x 1.5 ml

Thermoprime ReddyMix™ MasterMix

Developed to minimize preparation time for the PCR reaction.
Combines the properties of ReddyMix to the effectiveness of the Thermoprime.
As MasterMix, available in multiple versions.

Taq DNA Polymerase Kit (Thermus aquaticus) 5 U/µl with dNTPs

• Recombinant protein, ultrapure, at an initial concentration of 5 U/µl
• Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity
• Adds a free adenine to the 3' end of the amplicon (monoadenylation)
• Kit with 3 different 10X reaction buffers: without MgCl₂ (A), with MgCl₂ (B), with 2 inert dyes for direct deposit (C)
• Kit supplied with dNTPs (separate tube)

Color Taq DNA Polymerase Kit (1 U/µl) with dNTPs

• Recombinant protein, ultrapure, at an initial concentration of 1 U/µl
• Contains 2 inert dyes (red and yellow) to facilitate PCR preparation and allow direct deposition on electrophoresis gel and migration monitoring
• Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity
• Adds a free adenine to the 3' end of the amplicon (monoadenylation)
• Kit with 2 different 10X reaction buffers: without MgCl₂ (A) and with MgCl₂ (B)
• Kit supplied with dNTPs (separate tube)

Fastgene Taq DNA Polymerase

• Highly purified polymerase by 3 different chromatography methods and very active
• Compatible with TA-cloning
• Concentration: 5 U/µl
• Supplied with 2 separate reaction buffers to choose from depending on the type of PCR and a tube of MgCl₂
• Long-term storage at -20 °C
• Available in 500 U and 2000 U sizes

Fastgene Taq READY MIX 2X

• PCR mix containing Fastgene Taq DNA polymerase (1 U per 50 µl reaction volume), dNTPs (0.2 mM final of each), MgCl₂ (1.5 mM final) and stabilisation factors
• Supplied with two inert dyes to allow direct deposition of PCR products on agarose gel

Fastgene BAC free HS Taq

• Recombinant Thermus aquaticus DNA polymerase, cloned, produced and purified from eukaryotic cells
• Can be used for screening bacterial genomes without false positive detection
• Compatible with multiplex PCR, RT-PCR and difficult amplifications

Master mix SG qPCR (2X) and Master mix SG qPCR (2X) with ROX solution

• SYBR Green I dye qPCR mix for use with most real-time thermal cyclers
• Contains Perpetual Taq hot start DNA polymerase, optimised buffer, dNTPs (dTTP partially replaced by dUTP) and SYBR Green I dye
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)
• Available with ROX dye (separate tube) for standardisation

Master mix Blank qPCR (2X) and Master mix Blank qPCR (2X) with ROX solution

• Dye qPCR mix for use with most real-time thermal cyclers
• Contains no dye to allow choice of dye and optimisation of its concentration
• Contains Perpetual Taq hot start DNA polymerase, optimised buffer and dNTPs (dTTP partially replaced by dUTP)
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)
• Available with ROX dye (separate tube) for standardisation

Fastgene 2X IC green universal qPCR kit with ROX

• Universal qPCR kit with IC Green fluorescent dye development
• Sensitive and robust
• Can be used for DNA quantification, gene detection, melting curve analysis and more
• Supplied with ROX solution in a separate tube for standardisation

Fastgene 2X IC green universal qPCR kit with Fluorescein

• Universal qPCR kit with IC Green fluorescent dye development
• Sensitive and robust
• Can be used for DNA quantification, gene detection, melting curve analysis and more
• Reference Description € excl. tax h Supplied with fluorescein solution for standardisation

Master mix Probe qPCR (2X) and Master mix Probe qPCR (2X) with ROX solution

• QPCR mix with hydrolysis probes for use with most real-time thermal cyclers
• Contains Perpetual Taq hot start DNA polymerase and optimised buffer and dNTPs (dTTP partially replaced by dUTP)
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)
• Available with ROX dye (separate tube) for standardisation

Fastgene 2X probe universal qPCR kit

• Universal qPCR kit for hydrolysis probes
• Suitable for multiplex qPCR and rapid qPCR protocols due to robust mix chemistry
• For use in DNA quantification, gene detection, SNP genotyping or NGS validation
• Supplied with ROX solution in a separate tube for standardisation
• Can be used on all real time thermal cyclers

Hi-fi Pop DNA polymerase

• For applications that require high fidelity such as cloning, sequencing, SNP analysis and mutagenesis
• 60 times higher fidelity than conventional Taq polymerase
• Amplification of long, GC-rich DNA sequences
• Complex matrices (very rich in GC, complex secondary structures, very long sequences, etc.) may require the addition of a 5M betaine enhancer (optional)
• High elongation rate: 10 sec/kb
• 3' -' 5' exonuclease activity