Taq polymerases, kits and master mixes -

Disco taq DNA polymerase

• High throughput and reliable amplification for routine applications, screening or high-throughput screening
• Amplification jusqu'à 5 kb, compatible avec le TA-cloning et l'incorporation de dUTP
• 5' -> 3' exonuclease activity and absence of 3'-> 5' exonuclease activity

Hot Disco Taq DNA polymerase

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• Inactivated at room temperature, activation at 95°C for 15 min
• Incorporation possible de dUTP
• High specificity and sensitivity for high performance
• Pour détection de cibles même en faible quantité, le screening, le mutliplexage, la PCR directe sur colonies, et les applications de qPCR

Samples available, please contact us!

Biodyne enzymes

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• All enzymes produced are stable at ambient temperature
• A specific genetic modification: "Stability TAG" increases polypeptide shelf-life and its tolerance for temperature at a wider scale without compromising the properties of the polypeptide itself
• After being stored at ambient temperature (tested up to +35 °C), the enzyme remains fully active

Advantages
• Easy Pre-PCR preparation, fast and without any particular precautionary measures (no need for ice bath)
• The best quality/price ratio on the market
• Reduced waste and environmental impact (no need for ice shipping, smaller packaging)

Taq DNA Polymerase Kit (Thermus aquaticus) 5 U/µl with dNTPs

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• Recombinant protein, ultrapure, at an initial concentration of 5 U/µl
• Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity
• Adds a free adenine to the 3' end of the amplicon (monoadenylation)
• Kit with 3 different 10X reaction buffers: without MgCl₂ (A), with MgCl₂ (B), with 2 inert dyes for direct deposit (C)
• Kit supplied with dNTPs (separate tube)

Color Taq DNA Polymerase Kit (1 U/µl) with dNTPs

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• Recombinant protein, ultrapure, at an initial concentration of 1 U/µl
• Contains 2 inert dyes (red and yellow) to facilitate PCR preparation and allow direct deposition on electrophoresis gel and migration monitoring
• Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity
• Adds a free adenine to the 3' end of the amplicon (monoadenylation)
• Kit with 2 different 10X reaction buffers: without MgCl₂ (A) and with MgCl₂ (B)
• Kit supplied with dNTPs (separate tube)

Fastgene Taq DNA Polymerase

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• Highly purified polymerase by 3 different chromatography methods and very active
• Compatible with TA-cloning
• Concentration: 5 U/µl
• Supplied with 2 separate reaction buffers to choose from depending on the type of PCR and a tube of MgCl₂
• Long-term storage at -20 °C
• Available in 500 U and 2000 U sizes

Cytiva PCR components

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Taq DNA Polymerase (cloned): 1 unit incorporates 10 nmol of total nucleotides into acid-insoluble material in 30 min at 70ºC, in a total volume of 50 µl.

Cytiva illustra™ PCR kits: Ready-To-Go™ technique

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PuReTaq Ready-To-Go PCR Beads are pre-mixed, predispensed, single-dose reactions optimised for performing standard PCR amplifications.

Biodyne HOT FIREPol®

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FastGene Optima

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Mixture of 2 highly purified polymerases for the amplification of difficult samples and very long amplicons.

Cytiva PCR illustra™ kits: Ready-To-Go™ technique

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The RTG (Ready-To-Go) is to provide the mix necessary for the reaction as preformulated, premixed, predispensed beads

• Increased reproducibility
• Minimized pipetting steps
• Reduced the potential for contamination
• Long-term ambient-temperature stable (> 12 months)

Illustra Hot Start Mix RTG beads: for PCR requiring high amplification specificity. The only reagents to be added are water, the DNA to amplify and the primers.

Polymerase Chain Reaction (PCR) is covered by patents held by Roche Molecular Systems and F Hoffmann-La Roche Ltd.