Molecular Biology - Nucleic Acids DNA Purification and selection Compatible NGS or Sanger

Compatible NGS or Sanger

AxyPrep MAG Kit, optimised fragment selection chemistries for Illumina, SOLiD and Roche

• Unique paramagnetic bead technology for optimised DNA size selection suitable for NGS Illumina, Solid and Roche platforms
• 2 stages process:
- 1st step: determine AxyPrep Mag FragmentSelect-I ratio to DNA sample (optimal concentration) for target DNA size selection
- 2nd step: use the determined AxyPrep Mag FragmentSelect-I ratio for insulation of target DNA fragment size

Benefits:
• Economic: DNA fragment ranging from 200 bp to 1 kb
• Simple: versatile and flexible sequential fragment size selection: no gels needed
• Efficient: higher enrichment rate >60 %

AxyPrep™ Mag PCR Normalizer Kit

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• Allows for an identical concentration of amplicons ahead of NGS
• Beads binding capacity: 200 ng for 10 µl

Benefits:
• Dual action: Normalization and PCR clean up enables significant reduction in processing and faster turn around times
• Customisation available upon request

AxyPrep™ Mag DyeClean-Up Kit

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• Clean-up of excess dye terminator post sequencing reaction in Sanger sequencing
• Protocole: binding, washing and elution
• Can be performed directly in the thermal cycling plate
• No BigDye signal enhancer buffer required
• High signal to noise ratio resulting in long reads and high QV20+ scores
• Suitable for use with ABI Prism 3730, 3730 xl, 3700, 3130, 3130 xl and 3100 and Cytiva MegaBACE
• Simple and easy: 25 minutes/96 samples
• No centrifugation or filtration required