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Molecular Biology - Nucleic Acids DNA Restriction / Modification Ligases

Ligases

T4 DNA Ligase

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• Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA
• Catalyse la ligation de 2 fragments d'ADN double brin à bouts francs
• Covalently joins DNA fragments with complementary cohesive ends
• Seals single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids
• Suitable for cloning of restriction fragments and joining linkers or adapters to blunt-ended DNA
• Available in 20000 and 100000 unit formats*
• *Cohesive End Unit - 200 CEU = 3 Weiss units

Ready-to-go T4 DNA ligase

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Single-dose lyophilised reaction mix. Ambient-temperature storage. For covalently-linking DNA fragments in 30 min.