Sanger sequencing -

BrilliantDye™ Terminator 2X Cyclic Sequencing Kit

• Ready-to-use reagents for single- and double-stranded DNA sequencing by Sanger method
• For all types of DNA sequencing applications, including de novo sequencing and resequencing

• Contains all the necessary premixed reagents: BrilliantDye Terminator reagent, 5X sequencing buffer, pGEM control, M13 control primers
• 3 versions available:
- v1.1: Optimal resolution of the base calling adjacent to the primer
- v3.1: optimised for long sequences
- dGTP v3.1: optimised for sequences rich in G or GT
• Compatible with ABI 310, 3100, 3130, 3500, 373 and SeqStudio (Flex) sequencers, without protocol or calibration modifications

NimaPOP™ Polymer

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3 polymer types:
• NimaPOP™-4: Fast turnaround time. Forensic application (human identification)
• NimaPOP™-6: Ideal for long runs and for reading bases close to the 5' end
• NimaPOP™-7: Versatile, designed for a wide range of applications

Available formats :
• 5 and 10 ml bottle for 3130 series sequencers*
• 28 ml bottle for 3730 series sequencers*
• 5 or 10ml bottle for 310/3100 series sequencers*
• 384 or 960 sample bag with RFID chip for 3500 series and SeqStudio™ Flex sequencers*
* No protocol or calibration changes required

NimaPOP™ 10X migration buffer

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• Compatible with all polymers
• Supplied with RFID tags for anodic and cathodic buffer containers
• No protocol or calibration changes required
• Compatible with 310, 3100, 3130, and 3730 series sequencers

NimaPrime™ conditioning reagents

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• Primes and cleans the polymer pump to extend its life
• Recommended when changing polymer use or for routine maintenance
• Compatible with 3500 series and SeqStudio sequencers

ReCAP™ capillary regeneration kit

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• For maintenance and revitalisation of capillaries
• Complete cleaning of pump channels, polymer block and tubing
• Improves resolution and reduces "smears" and "tails" artefacts on the electrophoregram
• Eliminates background noise caused by residual yellow and red fluorophores
• Kit contains everything you need to regenerate a capillary network using the instrument's built-in programme

Fluorescent size marker

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• Fluorescent-size internal standard for 3130, 3500, 3730 and SeqStudio series sequencers
• Two versions:
- 600 bp : 22 fragments in length : 45, 72, 98, 128, 147, 167, 197, 227, 247, 267, 297, 327, 347, 367, 397, 427, 445, 465, 495, 525, 545, 565 and 595 bp
- 500 bp : 16 fragments marked with a red dye, length : 35, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490 and 500 bp
• Available with red (ROX™) or orange (LIZ™) coloured fragments