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Molecular biology - Proteins Marking and detection - Western blotting Secondary Antibodies

Secondary Antibodies

Labeling and detection for protein / Western Blotting / secondary antibodies

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Amersham ECL protein marking and detection for Western Blot

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• Sensitivity: 10 pg
• Duration of the program: 1 to 2 hours
• Reprobing possible with 2 antibodies
• Membrane surface: 60 000 cm²

HRP anti-IgG antibodies

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• Horseradish peroxidase-conjugated secondary antibodies to IgG
• Available in counter-adsorbed versions against bovine, horse, human, pig and rabbit serum proteins
• Counter adsorption increases antibody specificity by minimising cross-reactivity, increases sensitivity and reduces background staining

HRP anti-IGG antibody, Amersham

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• For Western blotting
• Optimised for use in conjunction with Amersham ECL detection reagents
• Antibodies to IgG conjugated to horseradish peroxidase
• Classical form (full antibody) or F(ab')2 fragment
• F(ab')2 fragment form eliminates aspecific interactions of the Fc moiety, facilitates cell penetration, no interference with anti-Fc antibodies