Molecular biology - Proteins Marking and detection - Western blotting Chemiluminescence - fluorescence

Chemiluminescence - fluorescence

Amersham ECL™, ECL Start™, ECL Advance™, ECL Select™, ECL Prime

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QuickStain staining kit for Proteins (Cy5) quantification

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• Allows fluorescence detection of Proteins directly after SDS-PAGE or transfer
• Excitation and emission wavelength:: 650 nm / 670 nm
• Up to 150 samples
• qualitative or quantitative marking according to staining time
• Does not require discolouration step
• Compatible with pre-cast gels
• Compatible with chemiluminescence or fluorescent secondary marking
• Without epitopes saturation for western blotting
• Low background noise

Reblot solutions

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These kits contain all the reagents for eliminating antibodies after chemiluminescence.

Merck Millipore Immobilon Western Chemiluminescent HRP Substrate

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• High sensitivity
• Wide range of sensitivities reducing the amount of antibody used
• Signal lasts for over 2 hours

Stable work Solution for 10 days

Luminata™ HRP substrate

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• Chemiluminescent reagents premixed for HRP detection by western blotting
• No risk of error in pipetting, ready to use
• The reagents have been matched to optimize the range and sensitivity of detection using various antibodies in Western blot assays. Stable at room temperature or at 4 ° C

Luminata Elisa HRP substrate

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• Chemiluminescent HRP detection reagent for ELISA's
• Requires a plate reader
• High sensitivity for detecting up to 0.1 pg protein
• The reagent has been matched to optimize the range and sensitivity of detection using various antibodies in ELISA assays
• Flexible use, with two types of substrates, Luminata crescendo and Forte