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Molecular biology - Proteins Sample preparation Extraction and splitting buffers

Extraction and splitting buffers

Cytiva Illustra™ Triple Prep Kit: simultaneous treatment of gDNA, RNA, and proteins from the same sample

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• Flexible, easy-to-follow workflow
• High yield resulting in up to 70% time saving
• Optimum workflow, minimal change of centrifugation speed, time, and pipetting volume
• Allows researchers to correlate data generated from the same sample
• High quality

Procedure without lysis step: 45 to 55 min.
Procedure with lysis step: 50 to 85 min

Cytiva protein sample preparation: lysis / protein extraction

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2-D Protein Extraction Buffer for gel analysis: a convenient way to prepare high quality protein lysates.

• Allows high quality results for downstream applications such as 2-D gel analysis
• Can also be used prior to 1-D electrophoresis, or for rehydrating IPG strips prior to 2-D electrophoresis
• Supplied as a dry powder which is rehydrated with the provided diluent
• Trial Kit allows you to evaluate each extraction buffer to find the most suitable buffer for your sample

Grinding kit: for disrupting small tissue and cell samples. Uses 1.5 ml microcentrifuge tubes, grinding resin and disposable pestles.

High-Abundant Protein Depletion: high-Abundant Protein Depletion kits conveniently remove >95% HSA and >90% IgG from 10 to 15 µl human serum, prior to 2-D electrophoresis

Mammalian and Yeast protein extraction buffer: for efficient and gentle extraction of biologically active, total soluble proteins from mammalian and yeast cultured cells

• Replaces laborious methods
• High reproductibility
• Total extraction of soluble proteins
• Yeast buffer: suitable for preparation of approximately 10 ml yeast cell pellets suspension as single or as multiple smaller preps
• Mammalian buffer: suitable for preparation of approximatively 10 ml of cell pellets

2-D Protein Extraction Buffer

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• Ready-to-use buffers for total protein extraction from tissues and cell lines, for 2-D gel analysis
• Efficient and reproducible protein extraction with high yield
• 6 different buffers covering the wide range of protein diversity