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• Kit for rapid DNA extraction (8 minutes) followed by PCR amplification and gel deposition • Extraction d'ADN à partir d'échantillons divers (tissus de mammifères, feuilles, plumes, nageoires de poisson, salive,...) • Compatible with automated pipetting systems • Available in a classic version with Disco Taq enzyme, or in a Hot Start version with Hot Disco Taq enzyme
• High throughput and reliable amplification for routine applications, screening or high-throughput screening • Amplification jusqu'à 5 kb, compatible avec le TA-cloning et l'incorporation de dUTP • 5' -> 3' exonuclease activity and absence of 3'-> 5' exonuclease activity
• Inactivated at room temperature, activation at 95°C for 15 min • Incorporation possible de dUTP • High specificity and sensitivity for high performance • Pour détection de cibles même en faible quantité, le screening, le mutliplexage, la PCR directe sur colonies, et les applications de qPCR
• Master mix 2X ready to use, addition of matrix, primers and water only • Taq inactivé à température ambiante, activation à 95 °C pendant 15 min • Simultaneous amplification of several targets in a single tube • Haute spécificité et sensibilité pour un rendement élevé • Minimises the risk of artefacts due to possible aspecific polymerisation before the first denaturation step • Incorporation possible de dUTP • The 2X buffer contains Hot Disco Taq DNA polymerase, dNTPS, optimised buffer and 6 mM MgCl2
• For applications that require high fidelity such as cloning, sequencing, SNP analysis and mutagenesis • 60 times higher fidelity than conventional Taq polymerase • Amplification of long, GC-rich DNA sequences • Complex matrices (very rich in GC, complex secondary structures, very long sequences, etc.) may require the addition of a 5M betaine enhancer (optional) • High elongation rate: 10 sec/kb • 3' -' 5' exonuclease activity