• Reaction mixtures for RT-qPCR in one step
• Contains all components necessary for cDNA synthesis and probed qPCR (besides matrix, primers and probes) in a single tube
• Probe Kit optimized for singleplex and duplex (1 to 2 targets)
• Multiplex Probe Kit Optimized for Multiplex (up to 4 targets)
• Optimized for DNA and LNA probes
• Probe Kit contains ROX dye for standardization
Specificities
:
Multiplex Probe Kit consisting of three reagents• qPCR 5x Mix with HOT FIREPol DNA polymerase, reaction buffer, dNTPs (dUTP included), 15 mm MgCl₂ (final concentration 3 mm)
• SOLIScript 40x Mix with SOLIScript reverse transcriptase and RIBOGRIP ribonuclease inhibitor
• Molecular biology quality Water
• Mix also available with ROX or PURPLE dye for thermocyclers requiring a reference signal for data normalization