• Mix optimised for quantitative PCR with dye detection
• High specificity and sensitivity
• Ready to use: just add matrix, water and primers
• Stable for 30 days at room temperature, long-term storage at -20 °C
Specificities
:
• Ideal when the sequence to be amplified is GC-rich
• High sensitivity even when the starting target is at low concentration
• Reduced risk of primer dimer formation
• Contains a blue dye to make pipetting easier
• Hot start enzyme inactivated at room temperature, activation in 12 minutes at 95°C
• Supplied with dUTPs to prevent cross-contamination after treatment with UNG (not supplied)
• Contains: Hot FIREPol® DNA polymerase, 5X qPCR buffer with 12.5 mM MgCl2 (final concentration 2.5 mM), dNTPs (including dUTP), EvaGreen® dye, GC enhancer and blue dye
• Compatible with most ROX dependent and independent thermal cyclers
