EURx (cloning) -

Universal Genomic DNA Extraction Kit

• Method based on the ethanol precipitation of nucleic acids obtained from cells or tissues lysed with GeDI reagent.
• The GeDI reagent is a single-phase solution containing no organic solvents
• Purified DNA can be used for common applications such as PCR, cloning, RFLP, Southern blotting, etc.
• Contents: GeDI reagent for total DNA isolation from various types of samples, ResSol Resuspension buffer, centrifuge columns with silica membrane
• Applicable sample quantities: 50 mg of animal tissue, 50 to 200 mg of plant tissue, 107 animal cells, bacteria or yeasts
• Yields vary according to the type of sample: 5 to 50 µg per 10 mg of animal tissue, 10 to 100 µg per 500 mg of leaf, 4 to 7 µg per 106 mammalian cells, 30 to 40 µg per 109 bacterial cells
• Available in 25 preparations and 100 preparations versions
• The GeDI reagent is also available in standalone version

RNA Fix, RNA stabilization reagent

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• Reagent for rapid stabilization and protection of RNA from fresh and non-frozen samples (tissues, leukocytes, cell or bacterial culture)
• Preserves RNA for up to 7 days at room temperature and up to 4 weeks at 4-8 °C, preservation for longer periods at -20 °C or -80 °C
• 10 volumes of reagent per volume of sample, or 10 µl per mg of sample

RNA Extracol

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• Total RNA extraction reagent from various samples (animal and human tissues, plants, cells, yeasts and bacteria)
• Phenol and guanidinium thiocyanate compounds
• Simple protocol, RNA extraction in one hour
• High efficiency
• Adjustable to material quantity
• Requires the addition of chloroform, isopropanol, ethanol and molecular biology grade water

Restriction endonucleases

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The majority of the restriction enzymes produced by EURx work in the ONE BUFFER buffer and can be used for double digestions.
The ONE BUFFER 10X is supplied with the enzyme.

T4 DNA Ligase

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• Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA
• Catalyse la ligation de 2 fragments d'ADN double brin à bouts francs
• Covalently joins DNA fragments with complementary cohesive ends
• Seals single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids
• Suitable for cloning of restriction fragments and joining linkers or adapters to blunt-ended DNA
• Available in 20000 and 100000 unit formats*
• *Cohesive End Unit - 200 CEU = 3 Weiss units

Alkaline phosphatase (CIP)

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• Catalyzes the hydrolysis of phosphate monoesters
• Can be used to remove the 5'-P end of DNA or RNA prior to 5' labelling
• Used to remove the 5'-P ends of linearized vectors to prevent their "empty" recircularization during cloning processes
• Compatible with protein dephosphorylation
• Available in 1000 and 5000 unit formats

Kinase T4 polynucleotide (PNK)

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• Catalyzes the phosphorylation of 5′ hydroxyl termini of double- and single-straned DNA or RNA
• Catalyzes the transfer of the γ-phosphate of ATP to a 5′-OH terminus in DNA or RNA
• The enzyme phosphorylates synthetic linkers and fragments of DNA or RNA prior to ligation
• Used for 5′-end labeling of nucleic acids prior to DNA or RNA sequencing
• Contains 3′-phosphatase activity
• Available in 1000 and 5000 unit formats

PfuPlus! DNA Polymerase

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• Mixture of DNA polymerase Pfu (Pyrococcus furiosus) and a thermostable polymerization activator
• Ultrapure, thermostable and proofreading recombinant polymerase
• Formulation designed to synthesize DNA sequences up to 20 kb
• More than 10 times more accuracy than a standard Taq
• Recommended for high-fidelity PCR, GC-rich sequences or sequences with problematic secondary structures, directed mutagenesis and blunt end cloning
• Can also be used for conventional PCR and high temperature primer extension
• Supplied with a 10X reaction buffer
• Available in 100, 500 and 2500 unit formats
• OnPfuPlus! DNA polymerase: hot start version, stable at room temperature, activation in 10 min at 90 °C

Exo-BAP Mix

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• Mix used for cleaning PCR products by enzymatic action prior to SNP analysis or sequencing
• Allows the degradation of dNTP and primers not used during PCR and still present in the reaction mix
• Contains heat-sensitive bacterial alkaline phosphatase (BAP) and exonuclease I in a specially formulated buffer
• Available in 100 reactions and 500 reactions formats

Thermosensitive bacterial alkaline phosphatase (BAP)

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• Very suitable for diagnostic immunodetection techniques and proteins immunolabelling and nucleic acids transferred on membrane techniques
• Catalyses the release of 5'-P and 3'-P groups of DNA, RNA and nucleotide molecules
• Removes the 5'-P from DNA, RNA, rNTP and dNTP molecules
• Used to remove the 5'-P ends of linearized vectors to prevent their "empty" recircularization during cloning processes
• Resistant to chemical changes and active in a wide variety of reaction buffers
• Can be inactivated by heat for 5 minutes at 70 °C
• Compatible with protein dephosphorylation
• Available in 1000 and 5000 unit formats

Exonuclease I

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• Catalyses the removal of nucleotides from single-stranded DNA in the 3' -> 5' direction
• Does not degrade double-stranded DNA or RNA
• Ideal for single-strand primers degradation after PCR prior to DNA sequencing or SNP analysis
• Used for single-stranded DNA degradation in a double-stranded DNA preparation
• Active in a wide variety of reaction buffers
• Available in 4000 and 20000 units formats

DNase I (RNase-free)

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• Endonuclease non-specifically degrading single and double stranded DNA by releasing di-, tri- and phosphorylated oligonucleotides in 5'.
• Used to remove contaminant DNA from RNA preparations prior to RT-PCR and RT-qPCR applications
• Also used to remove the DNA matrix from the reaction medium following in vitro transcription
• Available in 1000 and 5000 unit formats

RNase A (DNase-free)

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• Specific pyrimidine endoribonuclease that degrades single-stranded RNA
• Catalyses the cleavage of the phosphodiester bond between a pyrimidine and the following nucleotide
• Used for the suppression of RNA in DNA preparations
• Available in 10 and 50 mg formats

T7 Endonucléase I

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• It is structure-selective enzyme that recoginzes and cleaves mismatched DNA, heteroduplex DNA, cruciform DNA structures, Holliday structures or junctions and more slowly, nicked dsDNA
• The cleavage site is the first, second or third phosphodiester bond that is 5' to the mismatch
• Ultrapure recombinant enzyme

Possible applications:
• Recognition of mismatched DNA in particular in the context of genome editing using the Crispr method
• Resolve four-way junction or branched DNA
• Detect or cleave heteroduplex and nicked DNA
• Available in 250 and 1250 unit format