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Molecular biology Specialty ranges of molecular biology reagents Macherey-Nagel

Macherey-Nagel

RNA extraction kit from tissue or cell culture NucleoMag Macherey-Nagel

• Kit for the purification of RNA from bacteria, yeast, cell cultures or tissue
• Magnetic bead extraction
• Kits compatible with automatic purification systems
• For sample quantity < 2 x¹⁰⁶ eukaryotic cells or < 20 mg human or animal tissue
• For fragment sizes > 200 bp
• Yield up to 30 µg

NucleoMag Macherey-Nagel kit for the extraction of DNA and viral RNA from biological fluids

• Kit for the purification of viral DNA and RNA from biological fluids such as plasma or serum
• Magnetic bead extraction
• Kits compatible with automatic purification systems
• For sample quantity 200 µl
• For fragment sizes from 300 bp to 50 kpb

NucleoMag Macherey-Nagel kit for DNA and RNA extraction from water or air samples

• Kit for purification of DNA and RNA from water or air samples
• Magnetic bead extraction
• Kits compatible with automatic purification systems
• For sample quantities of 10 to 1000 ml
• For fragment sizes from 300 bp to 50 kbp

NucleoMag Dx Pathogen viral RNA extraction kit MACHEREY-NAGEL

• Kit for the purification of viral RNA from respiratory swab samples and fresh and untreated human saliva samples
• CE-IVD certified in vitro diagnostic medical device
• Magnetic bead extraction
• Kits compatible with automatic purification systems
• For sample quantity < 200 µl
• For fragment sizes from 300 bp to 50 kpb

NucleoSpin DNA extraction kit for tissues and organs MACHEREY-NAGEL

• Extraction of DNA from tissues or organs
• Extraction on silica membrane column by centrifugation
• For fragment sizes from 200 bp to 50 kbp
• Output from 1 to 30 µl

NucleoSpin MACHEREY-NAGEL water extraction kit for environmental DNA

• Environmental DNA extraction kit from water
• Extraction on column with silica matrix by centrifugation
• For sample quantity < 40 ml
• For fragment sizes from 100 bp to 50 kbp

NucleoZol MACHEREY-NAGEL

• Reagent for extraction of total RNA from various samples (cell culture, bacteria, yeast, plants)
• Yield of 1 to 8 µg of RNA per mg of sample
• Quick and easy extraction (1 hour)
• Does not require the use of chloroform
• Ready-to-use RNA for RT-qPCR, RNA chip, blotting and other applications
• Requires the use of ethanol, isopropanol and molecular biology grade water (not supplied)

Plasmid extraction kit for NucleoBond Xtra transfection MACHEREY-NAGEL

• Extraction of plasmid DNA for transfection from bacterial culture
• Anion exchange chromatography extraction 2nd generation by gravity
• For sample volumes of 40 ml to 1.2 l depending on the kit used

NucleoBond Xtra EF endotoxin-free plasmid extraction kit MACHEREY-NAGEL

• Extraction of endotoxin-free plasmid DNA from bacterial culture
• Anion exchange chromatography extraction 2nd generation by gravity
• For starting sample volume of 200 ml to 1.2 l depending on the kit used

NucleoBond PC EF endotoxin-free plasmid extraction kit MACHEREY-NAGEL

• Extraction of endotoxin-free plasmid DNA from bacterial culture
• Gravity anion exchange chromatography extraction
• For starting sample volume of 30 ml to 2 l depending on the kit used

Plasmid extraction kit for NucleoBond PC transfection MACHEREY-NAGEL

• Extraction of plasmid DNA for transfection from bacterial culture
• Extraction by gravity anion exchange chromatography
• For starting sample volume of 1 ml to 2 l depending on the kit used

MACHEREY-NAGEL NucleoSpin transfection plasmid extraction kit

• Extraction of plasmid DNA for transfection from bacterial culture
• Extraction on silica membrane column by centrifugation
• For starting sample volume of 1 to 5 ml

NucleoSpin and NucleoSpin EasyPure plasmid extraction kit MACHEREY-NAGEL

• Rapid extraction of plasmid DNA from bacterial culture
• Extraction on silica membrane column by centrifugation
• Ready-to-use DNA for restriction fragment analysis, cloning, PCR, sequencing and cell transfection
• NucleoSpin Plasmid EasyPure: very fast procedure with a single step of combined washing and drying
• For starting sample volume of 1 to 10 ml depending on the number of copies of the plasmid

NucleoSpin MACHEREY-NAGEL kit for DNA extraction from various samples

• DNA extraction from various samples (eukaryotic cells, bacteria, yeast, stool, urine, biopsies, bloodstains, hair, mouth swabs)
• Extraction on silica membrane column by centrifugation
• For fragment sizes from 200 bp to 50 kbp
• Yield of 20 to 35 µg

NucleoSpin plant DNA extraction kit MACHEREY-NAGEL

• DNA extraction from plant samples
• Extraction on silica membrane column by centrifugation
• Sample quantity:
- < 100 mg wet weight and < 20 mg dry weight for the Mini kit
- < 400 mg wet weight and < 80 mg dry weight for the Midi kit
- < 1500 mg wet weight and < 300 mg dry weight for the Maxi kit
• For fragment sizes from 50 bp to 50 kpb
• Performance:
- 1 to 30 µg for the Mini kit
- 10 to 100 µg for Midi kit
- 50 to 300 µg for Maxi size kit

NucleoSpin food DNA extraction kits MACHEREY-NAGEL

• DNA extraction from various samples (processed foods, soy (milk and flour), chocolate, cereals, meat)
• Extraction on silica membrane column by centrifugation
• For sample quantity < 200 mg
• For fragment sizes from 300 bp to 50 kpb
• Yield from 0.1 to 10 µg

NucleoSpin blood DNA extraction kit MACHEREY-NAGEL

• DNA extraction from blood, plasma or serum
• Extraction on silica membrane column by centrifugation
• Sample quantity:
- 5 to 200 µl for Mini kit
- 0.2 to 2 ml for Midi kit
- 2 to 10 ml for Maxi size kit
• For fragment sizes from 50 bp to 50 kpb
• Performance:
- 4 to 6 µg for Mini kit
- 40 to 60 µg for Midi kit
- 200 to 300 µg for Maxi kit

NucleoSpin and NucleoSpin plus RNA and miRNA extraction kit MACHEREY-NAGE

• Extraction of RNA or miRNA from various samples (cell culture, bacteria, yeast, human tissue, animal cells, biological fluids, etc.)
• Extraction on silica membrane column by centrifugation
• NucleoSpin RNA Plus Kit, supplied with NucleoSpin column, for removal of genomic DNA avoiding DNase treatment
• For sample quantity < 5 x 106 cells, < 30 mg human or animal tissue, < 109 bacteria or < 108 yeast

DNA extraction kit from bacterial culture NucleoMag MACHEREY-NAGEL

• Kit for DNA purification from bacterial and yeast cultures
• Magnetic bead extraction
• Kits compatible with automatic purification systems
• For sample quantity < 40 mg of bacteria (108 to 109 bacteria) or < 30 mg of yeast
• Yield from 5 to 25 µg

NucleoMag tissue and cell culture DNA extraction kit MACHEREY-NAGEL

• Kit for DNA purification from bacteria, yeast, cell cultures or tissues Magnetic bead extraction
• Kits compatible with automatic purification systems
• For sample quantity < 106 cells or < 20 mg of human or animal tissue
• For fragment sizes from 300 bp to 50 kpb
• Yield up to 30 µg

NucleoMag plant DNA extraction kit MACHEREY-NAGEL

• DNA purification kit from plants
• Magnetic bead extraction
• Kits compatible with automatic purification systems
• For sample quantities of 20 to 50 mg dry weight
• For fragment sizes from 300 bp to 50 kpb
• Yield of 10 to 20 µg

NucleoMag DNA extraction kit for soil, stool and biofilm samples MACHEREY-NAGEL

• Kit for purification of DNA from samples such as soil, biofilm or stool
• Magnetic bead extraction
• Kits compatible with automatic purification systems
• For sample quantities of 50 to 200 mg

NucleoMag blood DNA extraction kit MACHEREY-NAGEL

• DNA purification kit from blood
• Magnetic bead extraction
• Kits compatible with automatic purification systems
• For sample quantity < 200 µl
• For fragment sizes from 300 bp to 50 kpb
• Yield of 2 to 8 µg

NucleoMag food DNA extraction kit MACHEREY-NAGEL

• DNA extraction from food samples
• Magnetic bead extraction
• Kits compatible with automatic purification systems
• For sample quantity < 200 mg
• For fragment sizes from 300 bp to 50 kpb
• Yield from 0.1 to 10 µg

Purification of PCR products and fragments from NucleoSpin MACHEREY-NAGEL gel strips

• Purification of DNA or RNA from agarose gels or polyacrylamide gels and purification of PCR products
• Silica membrane column purification by centrifugation
• For sample:
- < 400 µl PCR reaction mix, < 400 µg agarose gel for Mini kit
- < 4 ml of PCR reaction mixture , < 4 mg of agarose gel for Midi format kit
- < 10 ml of PCR reaction mixture , < 10 mg of agarose gel for Maxi format kit
• For fragment sizes from 50 bp to 20 kbp
• Elution volume:
- from 15 to 30 µl for the Mini kit
- from 200 to 400 µl for the Midi format kit
- 1 ml for the Maxi size kit

Macherey-Nagel NucleoMag PCR Product Purification Kit

• PCR product purification kit
• Magnetic bead purification
• Kits compatible with automatic purification systems
• For sample quantity < 50 µl
• For fragment sizes from 150 bp to 10 kpb
• Elution volume from 25 to 100 µl

NucleoMag NGS clean-up resin MACHEREY-NAGEL

• Nucleic acid purification and size selection kit for NGS library construction
• Magnetic bead purification
• Kits compatible with automatic purification systems
• For sample quantities of 50 to 150 µl
• For fragment sizes from 150 to 800 bp
• Elution volume of 10 to 100 µl

Protino Ni-NTA protein purification kit MACHEREY-NAGEL

• Purification of poly-histidine-tagged proteins by affinity chromatography
• For all types of proteins (small proteins, large protein complexes, proteins with low expression levels)
• Purification under native or denaturing conditions
• Matrix: 6% agarose suspension (cross-linked), Ni2+ loaded