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• DNA extraction from various samples (eukaryotic cells, bacteria, yeast, stool, urine, biopsies, bloodstains, hair, mouth swabs) • Extraction on silica membrane column by centrifugation • For fragment sizes from 200 bp to 50 kbp • Yield of 20 to 35 µg
• Extraction of DNA from tissues or organs • Extraction on silica membrane column by centrifugation • For fragment sizes from 200 bp to 50 kbp • Output from 1 to 30 µl
• DNA extraction from plant samples • Extraction on silica membrane column by centrifugation • Sample quantity: - < 100 mg wet weight and < 20 mg dry weight for the Mini kit - < 400 mg wet weight and < 80 mg dry weight for the Midi kit - < 1500 mg wet weight and < 300 mg dry weight for the Maxi kit • For fragment sizes from 50 bp to 50 kpb • Performance: - 1 to 30 µg for the Mini kit - 10 to 100 µg for Midi kit - 50 to 300 µg for Maxi size kit
• DNA extraction from blood, plasma or serum • Extraction on silica membrane column by centrifugation • Sample quantity: - 5 to 200 µl for Mini kit - 0.2 to 2 ml for Midi kit - 2 to 10 ml for Maxi size kit • For fragment sizes from 50 bp to 50 kpb • Performance: - 4 to 6 µg for Mini kit - 40 to 60 µg for Midi kit - 200 to 300 µg for Maxi kit
• DNA extraction from various samples (processed foods, soy (milk and flour), chocolate, cereals, meat) • Extraction on silica membrane column by centrifugation • For sample quantity < 200 mg • For fragment sizes from 300 bp to 50 kpb • Yield from 0.1 to 10 µg
• Environmental DNA extraction kit from water • Extraction on column with silica matrix by centrifugation • For sample quantity < 40 ml • For fragment sizes from 100 bp to 50 kbp
• Illustra bacteria genomicPrep Mini Spin Kit • For the rapid extraction and purification of genomic DNA from gram-positive and gram-negative bacteria • Total DNA extraction time under 1 h • Can be used to purify 4 to 8 µg of DNA for Gram + (from 2 x 109bacteria cells) • Kit designed for input amounts ranging from 1 × 109to 4 × 109bacteria cells • A260/A280 between 1.7 and 1.9 • Contains Proteinase K
• Illustra tissue and cells genomicPrep Mini Spin kit • For the rapid extraction of high molecular weight genomic DNA (gDNA) from tissues and cell cultures • Up to 1.5 µg DNA / mg of tissue from an input tissue sample range of 5 to 50 mg • Up to 40 µg genomic DNA for 5 millions cells (depending on cell type) • Total DNA extraction in 90 min • A 260/ A 280 = 1.8
• Illustra blood genomicPrep Mini Spin Kit • For the rapid extraction and purification of DNA from whole blood, buffy coat, bone marrow, and nucleated red blood cells • Excellent repeatability and optimal yield (5 to 10 µg DNA from a 200 µl sample) • Input sample volumes can vary from 50 µl to 1 ml • DNA purity: A 260/A 280 >1.6 • Total time of preparation: 20 min (includes lysis, purification and desalting)
• GeneMATRIX is a new generation resin selectively binding nucleic acids to silica composite structures • Rapid Protocol • For High purity genomic DNA, free of any contaminant (RNA, proteins, lipids, dyes, detergents, organic enzyme inhibitors, buffers, salts, divalent cations, etc.)...) • DNA ready for use at the end of the procedure thanks to a low-salt elution buffer
• Genomic DNA extraction from eukaryotic cells • Starting material: tissues, cells, rodent tail, buccal swaps and paraffin samples • Fast and simple procedure • Extraction time: approximately 8 min after lysis • Binding capacity: 100 µg DNA • Pure DNA, ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing
• Uses columns • Extraction performed in 30 minutes • Max. DNA: 10 ug • Recovery of 60 to 90% of DNA for fragments up to 50 kb • A 260 / A280 between 1.7 and 2
• DNA extraction from plant material (fresh, frozen, leaves, roots, buds and flowers) • Pre-filtration to get rid of non-lysed material • 3 lysis buffers available in the kit to find the optimal conditions depending on the type of tissue • Quick and Simple • Extraction / Purification: approx. 40 min • Binding ability of the column: approx. 50 µg DNA • Pure DNA ready to be used for all enzymatic reactions, sequencing
• Extraction of DNA from bacteria (Gram + or -) • Extraction/Purification: approx. 45 min. • Quick and simple • Combined action of lysozyme and a proteolytic lysis • Binding capacity of the column: 50 micrograms DNA • Pure DNA ready to be used for all enzymatic reactions, sequencing